Coding

Part:BBa_K4692002

Designed by: Shamlan Reshamwala   Group: iGEM23_ICT-Mumbai   (2023-10-10)


Phytase from Aspergillus niger

This is a protein-coding part, coding for a phytase from Aspergillus niger BCC18081. This gene has been codon-optimized for expression in Saccharomyces cerevisiae. This phytase has been described in an article by Promdonkoy et al. (FEMS Microbiol Lett 2009 290(1):18-24). Optimal phytase activity is at 50 degrees C and pH 5.5. The enzyme is highly thermostable, as it retains >50% activity at 100 degrees C for 10 min. The phytase exhibits broad pH stability from 2.0 to 8.0.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 679
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None